中国水产科学2011，Vol.18Issue(6)：1259-1268,10.DOI:10.3724/SP.J.1118.2011.01259
真鲷精子诱导漠斑牙鲆减数雌核发育
Induction of diploid gynogenesis in southern flounder (Paralichthys lethostigma) with heterologous sperm of Pagrosomus major
摘要
Abstract
Female southern flounder (Paralichthys lethostigma) grow faster than males. Therefore, farming all-female progenies could maximize profits from cultured fish. In the present study, diploid gynogenesis was induced by activating flounder egg development with UV-irradiated (72 mJ/cm2) Pagrosomus major sperm for 3 min in sea water, and then subjecting the eggs to a cold-shock (0-2℃) treatment for 45 min. Cold shock was used to prevent extrusion of the second polar body. In the control treatments, one or more of these steps was omitted to separately assess the effectiveness of UV irradiation and cold shock. When the initial treatment was applied 3 min after hatching (treatment duration, 45 min), the fertilization rate of eggs activated by UV-irradiated heterologous sperm was (37.2 ± 5.1)%, the malformation rate of newly hatched larvae was (8.3 ± 2.5)%, and the hatching rate was up to (9.4 ± 0.71)%. Flow cytometry and chromosome karyotyping were used to examine the ploidy level of larvae experienced to the cold-shock treatment. All of the gynogenetic larvae were diploid (2n = 48), and no haploid or aneuploid phenomena were detected. The duration of hatching of haploid embryos and gynogenetic embryos was significantly different from that of control normal embryos. There were no significant differences in the morphological index between gynogenetic diploids and normal larvae (P>0.05). All of the haploid larvae (100%) showed abnormal external morphology (P<0.05) while normal diploid larvae and gynogenetic diploid larvae showed normal morphologies. In summary, these methods resulted in successful diploid gynogenesis in southern flounder using heterologous sperm. This technique could serve as a useful tool to produce monosex progenies of southern flounder.关键词
漠斑牙鲆/异源精子/雌核发育/冷休克/胚胎发育Key words
Paralichthys lethostigma/ heterologous sperm/ gynogenesis/ cold shock/ embryonic development分类
农业科技引用本文复制引用
柳学周,宁鑫,徐永江,赵明,李春广,李军..真鲷精子诱导漠斑牙鲆减数雌核发育[J].中国水产科学,2011,18(6):1259-1268,10.基金项目
国家863计划项目(2010AA 10A402) （2010AA 10A402）
公益性农业行业专项项(200903005) （200903005）
国家鲆鲽类产业技术体系项目(CARS-50) （CARS-50）
青岛市成果专项项目(09-2-3-15-chg). （09-2-3-15-chg）
