中国水产科学2011，Vol.18Issue(6)：1278-1285,8.DOI:10.3724/SP.J.1118.2011.01278
鸭绿江水系唇(鱼骨)胚胎发育、仔鱼饥饿及其不可逆点
Embryonic development, experimental starvation, and the point of no return of Hemibarbus labeo (Pallas) in YaLu River
摘要
Abstract
Hemibarbus labeo Pallas in the Yalu River and its tributaries is a promising and emerging aquaculture population in northeast China. However, the wild population of H. Labeo in the river has declined recently. We studied embryonic development, the effects of starvation, and the point of no return (PNR) of H. Labeo brood-stocks selected from the Yalu River and its tributaries. Three batches of fertilized eggs were produced by artificial spawning. Mature eggs were spherical, ecru, and (1.94±0.13) mm in diameter. Fertilized eggs were spherical, adhesive, and (3.07±0.12) mm in diameter. At 14-24.5°C, normal embryonic development using 94 h and 2 min to progress through one-cell, cell division, blastula, gastrula, neurula, and organ formation stages. Newly-hatched larvae were (8.07±0.27) mm in total length and the yolk sac was (0.55±0.12) mm3 in volume. The yolk sac had absorbed completely at 6 days post-hatch (dph). The total length and body weight of the control group (feeding 2 times everyday ) had increased to(12.58±1.03) mm and (0.010 8±0.002 2) g, respectively by 13 dph and by 24 dph to (17.21±0.99) mm and (0.023 7±0.005 4) g, respectively. Total length (R2=0.982 6) and body weight (R2=0.963 6) in experimental groups were both positively correlated to dph in the control group. In contrast, both total length and weight of the starved larvae began to decrease after absorption of the yolk sac at 6 dph, declining to (9.32±0.47) mm and (0.0022±0.0005) g, respectively, at 13 dph. Control larvae started to feed at 5 dph, at which time the feeding rate was 65%; 100% were feeding at 7 to 10 dph. Starved larvae suffered from PNR at 12-13 dph, and all died by 14 dph. Understanding embryonic development and knowing the effects of starvation will be important to artificially propagating H. Labeo and in fish ecology and fisheries biology. Our research will play a key role in artificially propagating large numbers of individuals to augment the wild population and its recruitment deficits.关键词
唇(鱼骨)骨/胚胎/饥饿/不可逆点Key words
Hemibarbus labeo Pallas/ embryo/ starvation/ point of no return分类
农业科技引用本文复制引用
骆小年,李军,刘刚,夏大明,金广海,吴瑞兰,杜华..鸭绿江水系唇(鱼骨)胚胎发育、仔鱼饥饿及其不可逆点[J].中国水产科学,2011,18(6):1278-1285,8.基金项目
辽宁省重大、重点项目“渔业新品种引进、开发”(20082030001). （20082030001）
