安徽农业科学2011，Vol.39Issue(31)：19054-19056,3.
带有组氨酸标签的蛇毒基因在毕赤酵母中的表达
Expression of A Snake Venom Gene with Histidine Tag in Pichia Pastoris
摘要
Abstract
[Objective] A snake venom gene with Histidine Tag was expressed through Pichia pastoris. [ Method] Using the gene recombination technology, with the sea snake as the material, a 6 × His Tag was inserted into the N end of the snake venom gene through the PCR technology and the restructured plasmid was transferred into the GS115 strains, after which was induced by 1% methanol, the recombinant protein was secreted and expressed. [ Result] The sequencing results revealed that DNA 6 × His Tag was accurately inserted into the expression vector, the purpose protein with the molecular weight of 28.5 was detected by SDS-PAGE. [Conclusion] The snake venom gene with Histidine Tag was expressed successfully and it had good defibre activity.关键词
蛇毒基因/毕赤酵母/分泌表达Key words
Snake venom gene/Pichia pastoris/Secretory expression分类
农业科技引用本文复制引用
黎卓键..带有组氨酸标签的蛇毒基因在毕赤酵母中的表达[J].安徽农业科学,2011,39(31):19054-19056,3.基金项目
广东省教育部产学研结合项目(2009B090300284). （2009B090300284）
