安徽农业科学2011,Vol.39Issue(31):19057-19058,2.
市售马铃薯转基因成分检测
Detection of Genetically Modified Potatoes from Market
摘要
Abstract
[Objective] The aim was to delect the genetically modified potatoes from market. [Method] By dint of CATB method, genomic DNA in the sample was extracted- The exogenous promoter and lerminator such as CaMV 35s (promoter from canliflomer mosaic virus) and NOS (nopaline synthase lerminator) were used as the detection primers to genetically engineered detect on Yinchuan city's commercial potato. PCR amplification on geaomic DNA was carried out. According to the PCR amplification product's agarose gel electrophoresis can analyze whether it contains genetically modified ingredients. [ Result] The results showed that: 50% of potato samples were detected to contain genetically modified ingredients. [Conclusion] Results indicated that 50% of potato included genetically modified ingredients in market.关键词
转基因食品/转基因检测/DNA提取/马铃薯Key words
Genetically modified food/ GM testing/ DNA extraction/ Potato分类
农业科技引用本文复制引用
韩学波,韩生银,马慧,王妮..市售马铃薯转基因成分检测[J].安徽农业科学,2011,39(31):19057-19058,2.基金项目
宁夏回族自治区区级大学生创新性实验荻批项目(宁教函(2010)381号). (宁教函(2010)