中国实验动物学报2011，Vol.19Issue(5)：433-436,4.DOI:10.3969/j.issn.1005-4847.2011.05.017
应用绿色荧光蛋白标记迟缓爱德华菌感染斑马鱼
Visualisation of zebrafish infection with GFP-labelled Edwardsiella tarda
摘要
Abstract
Objective To establish a zebrafish model of Edwardsiella tarda infection to study the pathogenicity and possible route of infection. Methods The infection process and histopathology were visualized in the zebrafish infected by E. Tarda labelled with green fluorescent protein. Results Histopathological observation revealed hydropic degeneration, atrophy and necrosis of liver cells, scattered hyperplastic nodules, congestion, edema and loss of lymphocytes in the spleen. The gastrointestinal tract was the first site where the pathogen was detected, followed by gill and skin. Conclusions Zebrafish can be used as an animal model for studying the pathogenicity of E. Tarda infection. The results of our study provide evidence that intestine, gill and skin may be the main route of infection by E. Tarda.关键词
迟缓爱德华菌/绿色荧光蛋白/斑马鱼/致病性/感染Key words
Edwardsiella tarda/ Green fluorescent protein/ Zebrafish/ Pathogenicity/ Infection分类
生物科学引用本文复制引用
王雪鹏,丁雷,邹兰柱,陈少波,谢起浪,闫茂仓..应用绿色荧光蛋白标记迟缓爱德华菌感染斑马鱼[J].中国实验动物学报,2011,19(5):433-436,4.基金项目
山东省优秀中青年科学家科研奖励基金(BS2011 HZ012) （BS2011 HZ012）
中国博士后基金(2011490162) （2011490162）
浙江省近岸水域生物资源开发与保护重点实验室开放基金(2010F30003) （2010F30003）
浙江省科技厅创新团队建设与人才培养项目(2009 F20009). （2009 F20009）
