大豆科学2011，Vol.30Issue(5)：731-737,7.
大豆过氧化物酶基因随机突变文库的构建
Construction of Soybean Peroxidase Gene Random Mutation Library
摘要
Abstract
In order to improve enzymatic activity of soybean peroxidase(SBP)and study the correlation between its primary structure and enzymatic activity,sequential error-prone PCR method was used on soybean peroxidase genes(sbp) for two times randon mutation. The second round error-prone PCR products and plasmid vectors pPICZα-A were digested by double enzymes. After the PCR products ligated into the vectors by T4DNA ligase,recombinant plasmids pPICZa-A-sbp were transformed into Escherichia coli DH5ct through calcium chlorid method. A random mutation library was constructed,and the capacity was about 1.77 ×106 cfu.关键词
大豆过氧化物酶/易错PCR/基因文库/随机突变/酶定向进化Key words
Soybean peroxidase/Error-prone PCR/Gene library/Random mutation/Directed evolution of enzymes分类
农业科技引用本文复制引用
曾家豫,郑群,廖世奇,王黎,袁红霞,杨海棠,张丽琼,张宁..大豆过氧化物酶基因随机突变文库的构建[J].大豆科学,2011,30(5):731-737,7.基金项目
甘肃省教育厅资助项目(0601-28,0501B-16) （0601-28,0501B-16）
甘肃省自然科学基金资助项目(096RJZA035) （096RJZA035）
甘肃省高分子材料重点实验室开放课题资助项目(KF-06-01). （KF-06-01）
