大豆科学2011，Vol.30Issue(5)：738-742,5.
大豆线粒体细胞色素b基因的克隆及系统分析
Cloning and Phylogenetic Analysis of Mitochondrial Cytochrome b Gene from Soybean ( Glycine max)
摘要
Abstract
In this study, mitochondrial DNA uncompleted sequence was used to design special prime of mitochondrial cyto-chrome b gene(cob)and obtained conservation domain of soybean cob gene by PCR amplification. The results showed that cob gene was consisted by 1 173 bp which encoded 391 amino acids,with 58.1% A +T and 41.9% G + C content. Southern blot analysis revealed that more than 2 cob gene copies were found in G. Max mitochondrial genome. Amino acid sequence and phy-logenetic analysis showed that cob was most similar to the dicotyledon,with 98% identity at the nucleotide level. In conclusion, cob gene is a potential hereditary marker for phyletic classification basing on its highly conservative property.关键词
大豆/线粒体/细胞色素bKey words
Soybean/Mitochondrial/Cytochromeb分类
农业科技引用本文复制引用
李玉秋,赵洪锟,刘晓东,张春宝,董英山..大豆线粒体细胞色素b基因的克隆及系统分析[J].大豆科学,2011,30(5):738-742,5.基金项目
国家转基因重大专项资助项目(2008ZX08004-004) （2008ZX08004-004）
国家自然科学青年基金资助项目(31000141) （31000141）
国家高技术研究发展计划重点资助项目(2006AA10Z120,2011AA10A105). （2006AA10Z120,2011AA10A105）
