大豆科学2011，Vol.30Issue(5)：752-756,5.
不同大豆基因型再生性及对农杆菌敏感性的研究
Regeneration and Sensitivity to Agrobacterium of Different Soybean Genotypes
摘要
Abstract
Sixteen soybean genotypes had been transformed by Agrobacterium-cotyledonary node system in this research. Differentiation ratio of multiple shoots and GUS positive rate as indicators, the regeneration of different soybean cultivars and their susceptibility to Agrobacterium tumifatiens were compared. Some factors affecting regeneration and genetic transformation such as 6-BA concentration in germination medium and PPT selection pressure were also studied. The result showed that germination medium supplemented with suitable concentration of 6-BA significantly increased the differentiation rate of buds; the sensitivity of PPT was different with varieties, the optimal concentration of PPT mainly concentrated in 5-7 mg ? L-1; Regeneration and sensitivity to Agrobacterium of 16 soybean genotypes were quite different, Hefeng 45 and Dongnong 50 were suitable genotypes for soybean transformation which had the highest regeneration rate and GUS expression.关键词
大豆/再生/农杆菌/敏感性Key words
Soybean/Regeneration/Agrobacterium/Susceptibility分类
农业科技引用本文复制引用
邱波,王志坤,孟凡立,李文滨..不同大豆基因型再生性及对农杆菌敏感性的研究[J].大豆科学,2011,30(5):752-756,5.基金项目
国家转基因作物新品种培育重大专项资助项目(2008ZX08004-002) （2008ZX08004-002）
国家自然科学基金资助项目(30800625). （30800625）
