天津科技大学学报2011，Vol.26Issue(5)：13-18,6.
一株来源于酒曲的纤溶酶产生菌的鉴定及其酶学性质
Identification of Producing Fibrinolytic Enzyme Strain from Starter and Its Enzymatic Properties
摘要
Abstract
A strain producing fibrinolytic enzyme was isolated from starter. The strain was identified as Rhizopus micro-sporus var. Tuberosus based on the morphological and ITS sequence. The enzymatic properties of the fibrinolytic enzyme secreted by the strain were determined. The properties tests suggest that the optimum temperature and pH were 37 °C and 7.0, respectively. The enzyme retained 95% of its initial activity by retaining for 4 h at 37 °C, and when it was treated at 57 °C for 4 h, the enzyme was still remained of activity. The enzyme had a wide range of pH adaptability, it still remained of part of activity when pH was adjusted to a level (pH<3.4); and it remained enzyme activity in between the range of pH 6.0-8.0. Zn2+ and Cu2+ have a very clear function to restrain the enzyme activity whereas Na+, Ca2+, Mn2+ showed obvious stimulation on the enzyme. The results show that the enzyme is stable in the artificial intestinal juice, it retained 80% of its initial activity by retaining for 4 h at 37 °C. It may be developed to injection used in clinical research.关键词
纤溶酶/菊芋小孢根霉/酶学性质Key words
fibrinolytic enzyme/ Rhizopus micrvsporus vai. Tuberosus/ enzymatic properties分类
生物科学引用本文复制引用
曹兴南,王海宽,孙岩,戚薇..一株来源于酒曲的纤溶酶产生菌的鉴定及其酶学性质[J].天津科技大学学报,2011,26(5):13-18,6.基金项目
天津市科技支撑计划项目(09ZCKFNC00800,10ZCKFNC01600) （09ZCKFNC00800,10ZCKFNC01600）
