大豆科学2011，Vol.30Issue(5)：813-817,5.
北美大豆猝死综合症病菌厚垣孢子的液体培养条件
Producing Conditions of Chlamydospore of Fusarium virguliforme sp.nov.in Liquid Culture
摘要
Abstract
The effects of culture medium,pH, temperature and oxygen on the chlamydospore production of Fusarium virguli-forme sp. Nov. Strain F-171 were studied by liquid culture method. The results showed that,the best liquid culture conditions for the chlamydospore production of the strain F-171 were,homogenized hyphae at 7500 r ? Min-1 for 2 min,filled 80 raL liquid culture of soybean stem or sprout juice in the 250 mL flask,and incubated at pH 6,25-30℃ and 120 r ? Min-1 shaking cu-litivation environment. Chlamydospore production curve in the best liquid culture conditions showed that the number of chlamydospore increased gradually with prolonging of the incubation time,reached the peak(3.15 ×105 spores ? mL-1) 10 days after incubation.关键词
北美大豆猝死综合症病菌/厚垣孢子/液体培养条件Key words
Fusarium. Virguliforme sp. Nov. (Chlamydospore/Culture condition分类
农业科技引用本文复制引用
师情,李会平,黄大庄,郭成亮,苏筱雨..北美大豆猝死综合症病菌厚垣孢子的液体培养条件[J].大豆科学,2011,30(5):813-817,5.基金项目
国家质检总局科技计划资助项目(2009IK264). （2009IK264）
