中国实验动物学报2011，Vol.19Issue(5)：419-422,4.DOI:10.3969/j.issn.1005-4847.2011.05.013
胎肝滤液诱导大鼠胎盘间充质干细胞向肝细胞的分化
Differentiation of rat placenta-derived mesenchymal stem cells into hepatocytes induced by fetal liver filtrate
摘要
Abstract
Objective To observe the differentiation of placental mesenchymal stem cells (PDMSCs) from E20d pregnant rats into hepatocytes induced by fetal liver filtrate, and explore the proliferation and differentiation conditions and induction methods of PDMSCs into hepatocytes cultured in vitro. Methods Placenta tissue was taken from the E20d pregnant rats under aseptic conditions. The placental tissue was digested by collagenase and centrifugation. The placenta-derived mesenchymal stem cells were isolated by adherence screening method and their surface antigen was identified. Fetal liver filtrate was added into the culture to simulate the environment in vivo and directionally induce PDMSCs to differentiate into hepatocytes. Immunocytochemistry was used to test the cellular markers, and PAS was used to detect glycogen expression. Results Under in vitro culture conditions, the PDMSCs developed into fibroblast-like cells, showing positive expression of CD44 surface marker. The cells appeared characteristically round and oval at 14 d when the PDMSCs were induced by fetal liver filtrate, and showing positive expressions of AFP and CK19. Conclusion Fetal liver filtrate can induce differentiation of PDMSCs into hepatocyte-like cells.关键词
胎肝滤液/胎盘间充质干细胞/诱导分化/大鼠/肝细胞Key words
Fetal liver filtrate/ Placenta-derived mesenchymal stem cells/ Differentiation/ Rat/ Hepatocytes分类
生物科学引用本文复制引用
王晓萃,吴金生,于树娜,蒋吉英,丁洁..胎肝滤液诱导大鼠胎盘间充质干细胞向肝细胞的分化[J].中国实验动物学报,2011,19(5):419-422,4.基金项目
山东省“泰山学者”建设工程专项经费. （）
