安徽农业科学2011，Vol.39Issue(31)：19224-19226,3.
实时荧光定量RT-PCR检测鱼类传染性胰脏坏死病病毒方法的建立
Establishment of Real-Time RT-PCR Assay for Detection of IPNV
摘要
Abstract
[Objective] The aim was to develop the detection of infectious pancreatic necrosis virus (IPNV)by real-time RT-PCR assay. [ Method] Primers and probe were designed using the software Primer Express 2.0. The plasmid containing the target sequence was constructed to detect the sensitivity and prepare the standard curve. The standard curve was prepared based on the linear relationship between the a-mount of plasmid DNA and cycle threshold ( Ct). [ Result] The linear relationship between virus concentration (X) and Ct was Ct = -3.069 6 +42.246 (R2 =0.999 8). The method was specific for IPNV and did not react with other comparative fish virus and nucleic acid of rainbow trout. [ Conclusion]The determination of IPNV by real-time RT-PCR with high sensitivity and specificity was an effective tool for the rapid detection of IPNV.关键词
传染性胰脏坏死病病毒(IPNV)/荧光定量RT-PCR/Taqman探针Key words
Infectious pancreatic necrosis virus (IPNV)/ Realtime RT-PCR/ Taqman probe分类
农业科技引用本文复制引用
徐晔,段宏安,周毅,刘亭歧,姚燕林..实时荧光定量RT-PCR检测鱼类传染性胰脏坏死病病毒方法的建立[J].安徽农业科学,2011,39(31):19224-19226,3.基金项目
国家质检总局质检公益项目(2011110037) （2011110037）
国家质检总局科技计划项目(2010IK014). （2010IK014）
