山西农业大学学报（自然科学版）2011，Vol.31Issue(4)：315-320,6.
文冠果DNA提取及RAPD反应体系的优化
Extraction of Xanthoceras Sorbifolia Bunge DNA and Optimization of the RAPD Reaction Conditions
摘要
Abstract
The RAPD reaction conditions of Xanthoceras sorbifolia Bunge in 20 counties in Shanxi province were optimized with the modified CTAB method for DNA extraction. The results showed that the optimal RAPD analysis reaction system as: The total volume of PCR amplification 20 piL, including 30 ng of template DNA, 10 X PCR buffer 2 μL, 2. 0 mmol · L-1Mg2+ , 0.1 mmol · L-1dNTPs, Taq enzyme 1 U. Amplification program is: 94°C pre-denaturation for 120 s, 94 °C denaturation 30 s, 36. 9°C annealing 45 s, 72°C extension 90 s. After 45 cycles , 72°C extension for 300 s, the end stored at 4°C. In optimum conditions, RAPD analysis has good stability and repeatability.关键词
文冠果/RAPD/体系优化Key words
Xanthoceras sorbi folia Bunge / RAPD/ System optimization分类
农业科技引用本文复制引用
高晓欣,张芸香,郭晋平..文冠果DNA提取及RAPD反应体系的优化[J].山西农业大学学报（自然科学版）,2011,31(4):315-320,6.基金项目
山西林业厅科技支撑项目(200809) （200809）
