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地肤内生细菌对番茄灰霉病菌的拮抗作用

谈静惠 康书静 郝晓娟 曹挥 王美琴 李生才 高俊明

山西农业大学学报(自然科学版)2011,Vol.31Issue(4):326-331,6.
山西农业大学学报(自然科学版)2011,Vol.31Issue(4):326-331,6.

地肤内生细菌对番茄灰霉病菌的拮抗作用

Antagonism of Endophyte Bacteria from Kochia scoparia on Botrytis cinerea

谈静惠 1康书静 1郝晓娟 1曹挥 1王美琴 1李生才 1高俊明1

作者信息

  • 1. 山西农业大学农学院,山西太谷030801
  • 折叠

摘要

Abstract

The aim of the study is to screen anti-microorganism endophytic bacteria for plant disease control. Five strains from the Kochia scoparia exhibited extensive antagonistic activities against Botrytiscinerea. D-29 strain showed strong antagonistic activity against Botrytis cinerea on tomato in vitro. The fermentation solution, suspension of bacteria and sterile filtrate could reduce the expension of leisons on red tomato in vitro and inhibitory rate reached 64.61%, 61. 50% and 44. 10% respectively. Fermentation solution could reduce the expension of leisons on green tomato in vitro and inhibitory rate reached 100% and 67. 11% in the 4d and 6d. The results of observation under microscope illustrated that D-29 could make cell material leaked and the deformity of top mycelium and spores infarction. 100% D-29 sterile filtrate had the most significant inhibitory effect anginst spore germination of Botrytis cinerea and its inhibiting rate reached 80%.

关键词

地肤/内生细菌/番茄灰霉病菌/拮抗作用

Key words

Kochia scoparia / Endophyte bacteria/ Botrytis cinerea / Antagonist role

分类

农业科技

引用本文复制引用

谈静惠,康书静,郝晓娟,曹挥,王美琴,李生才,高俊明..地肤内生细菌对番茄灰霉病菌的拮抗作用[J].山西农业大学学报(自然科学版),2011,31(4):326-331,6.

基金项目

国家自然基金青年基金(31000873) (31000873)

山西省青年基金(2010021031-1) (2010021031-1)

山西农业大学博士后基金 ()

山西农业大学科技创新基金(2009019) (2009019)

山西省科技攻关项目(20080311025) (20080311025)

山西农业大学学报(自然科学版)

OACSTPCD

1671-8151

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