安徽农业科学2011，Vol.39Issue(31)：19590-19593,4.
碱性蛋白酶水解文冠果蛋白酶的条件研究
Research on Hydrolysis Condition of Xanthoceras sorbifolia Protein by Alkali Protease
摘要
Abstract
[Objective]The paper aimed to study the optimum conditions of alkali protease hydrolyzing Xanthoceras sorbifolia protein. [ Method] Substrate concentration,pH value,temperature,enzyme dosage and hydrolysis time were selected as influence factors,and the degree of hydrolysis as evaluation value for the single factor analysis. Response surface methodology (RSM) was used to optimize the enzymatic hydrolysis conditions of alkali protease based on these factors. And the optimum conditions were subjected to verification test. [ Result]The optimum conditions were as follows: substrate concentration 5% .enzyme dosage 4% ,pH value 9.0. Under these conditions,the degree of hydrolysis was 19.75%. [ Conclusion] The paper could supply technical parameters for future researches and development of X. Sorbifolia protein.关键词
文冠果/碱性蛋白酶/水解/优化Key words
Xanthoceras sorbifolia/Alkali protease/Hydrolysis/Optimize分类
生物科学引用本文复制引用
杨永强,仇宏伟,赵四东,张涛,周泉城..碱性蛋白酶水解文冠果蛋白酶的条件研究[J].安徽农业科学,2011,39(31):19590-19593,4.基金项目
山东省教育厅科技发展计划项目(J08LF05). （J08LF05）
