水产学报2011，Vol.35Issue(10)：1458-1462,5.DOI:10.3724/SP.J.1231.2011.17227
KK-42对凡纳滨对虾热激蛋白基因表达的诱导作用
The induction of imidazole derivative KK-42 on heat shock protein expression in Litopenaeus vannamei
摘要
Abstract
Heat shock proteins(HSP) play important roles in coping with environmental stresses and self-protection in animals. Our previous study has demonstrated that the treatment of imidazole derivative KK-4,2 can increase the survival rate of Litopenaeus vannamei. In order to research the possible molecular mechanism of KK-42 effect on L. vannamei, a part of HSP90 mRNA sequence was first cloned; the spatial and temporal expressions of HSP90 and HSP70 as well as the effect of KK-42 on the expressions were determined. The shrimps(L. vannamei) ,3.5 -5.0 cm long, were soaked for 1 min in KK-42 solution at a concentration of 1.95 × 10 -4 mol/L or in the solution without KK-42, and then cultured in normal way. Results showed that HSP90 and HSP70 were expressed in mandibular organ, eyestalk, muscle and hepatopancreas tissues, of which the levels from hepatopancreas were higher. Compared to treatment group, hepatopancreas HSP90 and HSP70 mRNA levels were relatively lower although the obvious fluctuations of the levels were found during experiment in control group. Administration of KK42 could significantly induce hepatopancreas HSP90 and HSP10 expressions. The HSP70 and HSP90 mRNA contents increased by 243.4% ,141.3% ,410.5% and 121.6% ,505.5% ,481. 7% .respectively,compared with the corresponding controls on day 1, day 2 and day 3 after KK42 treatment. Our present study demonstrates that KK-42 treatment significantly induces the transcriptions of HSP90 and HSP70 from hepatopancreas, which is likely one of the mechanisms of promoting-survival effect of KK-42 on L. vannamei.关键词
凡纳滨对虾/KK-42/热激蛋白90/热激蛋白70Key words
Litopenaeus vannamei/ KK-42/ HSP90/ HSP70分类
生物科学引用本文复制引用
李昕,夏西超,宁黔冀..KK-42对凡纳滨对虾热激蛋白基因表达的诱导作用[J].水产学报,2011,35(10):1458-1462,5.基金项目
国家自然科学基金项目(30940008) （30940008）
高等学校博士学科点专项科研基金(20094104110003) （20094104110003）
河南省高校科技创新人才支持计划(2009HASTIT022) （2009HASTIT022）
