中国农业科学2011，Vol.44Issue(20)：4309-4316,8.
不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响
Effects of Different Immune Status on Blood Biochemical Parameters and Cellular Immune Response in Broiler Chickens
摘要
Abstract
[Objective] This experiment was conducted to determine the effects of different immune statuses on blood biochemical parameters and cellular immune response of broiler chickens. [Method] One hundred and eighty 1 -day-old AA broilers were allocated into four groups. Each treatment had 5 replicate cages of 9 broilers per replicate pen. This study will set different immune stress models, including the control group (no vaccines, NV), conventional vaccines group (CV), immune hyperthyroidism group (LPS) and immune suppression group (CYP). The T lymphocytes and subgroups were isolated from periphery blood from immunized and unvaccinated birds and were detected by CD3/CD4/CD8 triple labelling by flow cytometric analysis and MTT assays, respectively. [Result] The results showed that different immunological statuses had no significant effect on feed conversion ratio of broiler chickens among 4 treatment (P>0.05). LPS group and CYP group had significantly lower broiler body weight gain and feed intake than NV group (P<0.05). Serum TP, GLU, and CHLO contents were no significantly different among treatments (P>0.05). On d 21 and 35, ALB of LPS and CYP groups was significantly lower than CV and NV groups (P<0.05), and ALB levels of NV and CV groups were not significantly different (P>0.05). On d 21, a-AGP of LPS group was significantly lower than CV group (P< 0.05), BUN of LPS group was significantly higher than NV group (P<0.05). CPY and LPS inhibited the T, B lymphocyte proliferation, and CD4+/CD8+ T cell percentage of LPS group was significantly lower than NV group (P<0.05). [Conclusion] Chickens with no vaccines maintained normal growth performance and immune response. LPS stress increased the cellular immune response of chickens. Tolerance of chicken to CYP increased with age.关键词
AA肉鸡/免疫状态/血液指标/MTT法/淋巴细胞亚群Key words
AA broiler/ immune status/ blood parameters/ MTT assay/ lymphocyte cluster引用本文复制引用
冯焱,杨小军,王筱霏,覃定奎,尹瑞卿,姚军虎..不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响[J].中国农业科学,2011,44(20):4309-4316,8.基金项目
国家自然科学基金(31001017) （31001017）
陕西省科技攻关项目(2009K01-02) （2009K01-02）
陕西省自然基金(2010JQ3002) （2010JQ3002）
陕西省饲料工程技术研究中心项目(2010ZDGC-02) （2010ZDGC-02）
动物营养学国家重点实验室项目(2004DA125184F0809) （2004DA125184F0809）
