首页|期刊导航|中华医学杂志（英文版）|Increased expression of granulocyte colony-stimulating factor mediates mesenchymal stem cells recruitment after vascular injury
中华医学杂志（英文版）2011，Vol.124Issue(24)：4286-4292,7.DOI:10.3760/cma.j.issn.0366-6999.2011.24.029
Increased expression of granulocyte colony-stimulating factor mediates mesenchymal stem cells recruitment after vascular injury
Increased expression of granulocyte colony-stimulating factor mediates mesenchymal stem cells recruitment after vascular injury
摘要
Abstract
Background Recent studies indicate that bone marrow-derived cells may significantly contribute to atherosclerosis,post-angioplasty restenosis and transplantation-associated vasculopathy.The responsible bone marrow (BM) cells and mechanisms regulating the mobilization of these cells are currently unclear.The purpose of this study was to investigate the expression of granulocyte colony-stimulating factor (G-CSF) on injured arteries and its effects on mesenchymal stem cells (MSCs) differentiation into vascular smooth muscle cells (VSMCs) in the process of vascular remodeling.Methods Balloon-mediated vascular injury was established in female rats (n=1O0) which received radioprotective whole female BM cells by tail vein injection and male MSCs through a tibial BM injection after lethal irradiation.The injured and contralateral carotid arteries were harvested at 3,7,14 and 28 days after treatment.Results Morphometric analysis indicated that intima to media area-ratio (I/M ratio) significantly increased at 28 days,0.899±-0.057 (P ＜0.01),compared with uninjured arteries.Combining fluorescence in situ hybridization (FISH) and immunohistochemical analysis showed that a significant number of the neointimal cells derived from MSCs,(45.2±8.5)％ at 28 days (P=0.01),compared with (23.5±6.3)％ at 14 days.G-CSF was induced in carotid arteries subject to balloon angioplasty (fold mRNA change=8.67±0.63 at three days,relative G-CSF protein=0.657±-0.011 at three days,P ＜0.01,respectively,compared with uninjured arteries).G-CSF was chemotactic for MSCs but did not affect the differentiation of MSCs into smooth-muscle-like cells.Conclusion Increased expression of G-CSF by injured arteries plays an essential role in contribution to recruitment and homing of MSCs to the site of the arterial lesion.关键词
mesenchymal stem cells/smooth muscle cells/granulocyte colony-stimulating factor/neointimaKey words
mesenchymal stem cells/smooth muscle cells/granulocyte colony-stimulating factor/neointima引用本文复制引用
ZHAO Yong,LIU Ying-xi,XIEShuang-lun,DENG Bing-qing,WANG Jing-feng,NIE Ru-qiong..Increased expression of granulocyte colony-stimulating factor mediates mesenchymal stem cells recruitment after vascular injury[J].中华医学杂志（英文版）,2011,124(24):4286-4292,7.基金项目
This study was supported by grants from the National Natural Science Foundation of China (No.30770899 to NIE Ru-qiong),the Natural Science Foundation of Guangdong Province (No.8151008901000072 to NIE Ru-qiong). （No.30770899 to NIE Ru-qiong）
