安徽农业科学2011，Vol.39Issue(32)：19663-19665,19670,4.
利用高效液相色谱和紫外分析监测儿茶素酯化过程中的含量变化研究
Study on the Changes of the Catechins Content in the Esterification Process by Dint of HPLC and UV Methods
刘丽 1霍清1
作者信息
- 1. 北京联合大学生物化学工程学院生物制药系,北京100023
- 折叠
摘要
Abstract
Catechins is widely present in plants in natural medicine. Modern scientific research shows that catechins can cure cardiovascular disease, cancer and other features. Through esterification, the introduction of the long carbon chain molecules can increase its hydrophilic and hydro-phobic balance. This experiment established a HPLC method for the determination of catechins content. The column of AgilentZorbaxSB-C18 (416 x 250 mm ,5 μm) was used. The mobile phase was composed of formic acid - water - acetic acid (15:84:1) and the flow rate was 0.9 ml/ min,with UV detection at 278 no. Measured retention time of catechins absorption peak around t =22 min,HPLC standard curve y =6 x 108x + 248 469,R2 =0.998 2,linear range of 0.003 24-0.006 43 mg; UV spectrophotometer method was established:Ethanol as a constant volume solvent. The absorbance of catechins was measured at 278 nm. Determination of catechins standard curve was y =0. 100 5x -0.040 1 ,R2 = 0.999 4, linear range was 0.05 -0.4 mg / ml. It is convenient to use UV - Vis spectrophotometer for the observation of catechins in the esterification. HPLC is applied to monitor the growth of esterification products and to calculate synthetic yield rate.关键词
儿茶素/酯化反应/高效液相色谱/紫外Key words
Catechins/Esterification/HPLC/UV分类
农业科技引用本文复制引用
刘丽,霍清..利用高效液相色谱和紫外分析监测儿茶素酯化过程中的含量变化研究[J].安徽农业科学,2011,39(32):19663-19665,19670,4.
