中华医学杂志（英文版）2011，Vol.124Issue(24)：4310-4315,6.DOI:10.3760/cma.j.issn.0366-6999.2011.24.033
Resveratrol-induced augmentation of telomerase activity delays senescence of endothelial progenitor cells
Resveratrol-induced augmentation of telomerase activity delays senescence of endothelial progenitor cells
WANG Xiao-bin 1ZHU Li 1HUANG Jun 2YIN Yi-gang 3KONG Xiang-qing 2RONG Qi-fei 2SHI Ai-wu 2CAO Ke-jiang2
作者信息
- 1. Department of Cardiology, Taizhou People's Hospital, Second Affiliated to Medical College of Yangzhou University, Taizhou,Jiangsu 225300, China
- 2. Department of Cardiology, First Affiliated Hospital, Nanjing Me dical University, Nanjing, Jiangsu 210029, China
- 3. Department of Cardiology, Taizhou People's Hospital, Second Affiliated to Medical College of Yangzhou University, Taizhou,Jiangsu 225300, China
- 折叠
摘要
Abstract
Background Previous studies have shown that resveratrol increases endothelial progenitor cell (EPC) numbers and functional activity.Increased EPC numbers and activity are associated with the inhibition of EPC senescence.In this study,we investigated the effect of resveratrol on the senescence of EPCs,leading to potentiation of cellular function.Methods EPCs were isolated from human peripheral blood and identified immunocytochemically.EPCs were incubated with resveratrol (1,10,and 50 μmol/L) or control for specified times.After in vitro cultivation,acidic β-galactosidase staining revealed the extent of senescence in the cells.To gain further insight into the underlying mechanism of the effect of resveratrol,we measured telomerase activity using a polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay (ELISA) technique.Furthermore,we measured the expression of human telomerase reverse transcriptase (hTERT) and the phosphorylation of Akt by immunoblotting.Results Resveratrol dose-dependently inhibited the onset of EPC senescence in culture.Resveratrol also significantly increased telomerase activity.Interestingly,quantitative real-time PCR analysis demonstrated that resveratrol dose-dependently increased the expression of the catalytic subunit,hTERT,an effect that was significantly inhibited by pharmacological phosphatidylinositol 3-kinase (PI3-K) blockers (wortmannin).The expression of hTERT is regulated by the PI3-K/Akt pathway; therefore,we examined the effect of resveratrol on Akt activity in EPCs.Immunoblotting analysis revealed that resveratrol led to dose-dependent phosphorylation and activation of Akt in EPCs.Conclusion Resveratrol delayed EPCs senescence in vitro,which may be dependent on telomerase activation.关键词
endothelial progenitor cells/resveratrol/senescence/telomerase activityKey words
endothelial progenitor cells/resveratrol/senescence/telomerase activity引用本文复制引用
WANG Xiao-bin,ZHU Li,HUANG Jun,YIN Yi-gang,KONG Xiang-qing,RONG Qi-fei,SHI Ai-wu,CAO Ke-jiang..Resveratrol-induced augmentation of telomerase activity delays senescence of endothelial progenitor cells[J].中华医学杂志（英文版）,2011,124(24):4310-4315,6.
