安徽农业科学2011，Vol.39Issue(32)：19698-19699,19702,3.
木薯外植体表面灭菌与增殖培养的研究
A Study on the Sterilization and Proliferation of Cassava Explants in vitro
摘要
Abstract
[Objective] The theoretic reference for the cassava tissue multiplication was proviced through the research on the reasonable sterilization system. [ Method ] The axillary bud of cassava " GR891" was used as explants, which were processed by optimum concentration of Cer-cobinm or not. The effect of different sterilization methods and times on the sterilization results was studied. Simultaneously, the effect of 6-BA and the combination of 6 - BA and NAA on the shoot induction was also investigated. [ Result] The results demonstrated that the ideal effect was got when 75% alcohol was used in the treatment of axillary buds for 10 seconds and then 0. 1% mercuric chloride, for 8 minutes, which survival of explants was 61.3%. The highest frequency of shoot induction was from the MS medium supplemented with 1.5 mg/L of 6-BA + 30 g/L of sugar +6.5 g/L of agar, with 7-9 plantlets in single axillary bud, which grew fast and strongly. [ Conclusion] The system of explants sterilization and multiplication of cassava axillary bud was set up.关键词
木薯/GR891/外植体/表面灭菌/增殖培养Key words
cassava/GR891/explants/sterilization Proliferation training分类
农业科技引用本文复制引用
黎萍,彭靖茹,苏文潘,石兰蓉,李慧敏,黄惠芳,黄强..木薯外植体表面灭菌与增殖培养的研究[J].安徽农业科学,2011,39(32):19698-19699,19702,3.基金项目
广西科学研究与技术开发计划项目(主席科技基金10169-02) （主席科技基金10169-02）
广西科学研究与技术开发计划项目(桂科攻10123007-6A) （桂科攻10123007-6A）
广西科学研究与技术开发计划项目(主席基金09203-03). （主席基金09203-03）
