福建农林大学学报（自然科学版）2011，Vol.40Issue(6)：561-565,5.
红麻叶片高质量RNA提取方法比较分析
Comparison and analysis of methods of extracting high-quality total RNA from kenaf leaves
摘要
Abstract
RNA isolation from kenaf leaves is difficult due to containing large amounts of polysaccharides, polyphenol and other secondary metabolites, affecting both the quality and quantity of RNA. In this study, total RNA from the leaves of Fuhong 992 was extracted by the five methods of CTAB, hot-borate, SDS, Trizol and Biotake corporation kit, carried on the extraction effect comparative analysis. The results showed that the method of hot-borate was more suitable to isolate total UNA from kenaf leaves, next were CTAB and SDS these two methods, Trizol and Biotake corporation kit did not obtain the ideal result. It is capable of obtaining high quality and high integrity total RNA from kenaf leaves using hot-borate method, and conducting its reverse transcription to prepare cDNA. It demonstrated that the total RNA isolated by this method was of sufficient quality for subsequent molecular applications.关键词
红麻/叶片/总RNA提取Key words
Hibiscus cannabinw L / leaves/ total RNA extraction分类
农业科技引用本文复制引用
陈美霞,陈富成,颜克伟,刘晓倩,徐建堂,陶爱芬,祁建民..红麻叶片高质量RNA提取方法比较分析[J].福建农林大学学报（自然科学版）,2011,40(6):561-565,5.基金项目
国家麻类产业技术体系建设项目(nycytx-19-512) （nycytx-19-512）
国家自然科学基金项目(3100073). （3100073）
