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流苏石斛种子非共生萌发的研究

余乐 兰芹英 汤庚国

福建林学院学报2011,Vol.31Issue(4):346-348,3.
福建林学院学报2011,Vol.31Issue(4):346-348,3.

流苏石斛种子非共生萌发的研究

Study on nonsymbiotic sproution of Dendrobium fimbriatum Hook.

余乐 1兰芹英 2汤庚国3

作者信息

  • 1. 江苏畜牧兽医职业技术学院,江苏泰州225300
  • 2. 中国科学院西双版纳热带植物园,云南勐腊666303
  • 3. 南京林业大学森林资源与环境学院,江苏南京210037
  • 折叠

摘要

Abstract

Seeds of Dendrobium fimbriatum were sown aseptically for protcorms induced and organogenes, also the effects of different temperature, hormones combinations on the seed nonsymbiotic germination were studied. The results showed that protcorms, green and big, were induced after 7 - 15 d on KC basal medium with 2% sucrose, solidified with 0.7% agar at 25 ℃ and the seed germination rate maybe up to 98.3%. The actions of protcorm inducing and seeding differentiation were effective when they were cultured on KC medium +2 mg.L-1 6-BA +0.1 -0.5 mg.L-1 NAA with 3% sucrose , solidified with 0.7% agar at 25 ℃. The percentage of induction and proliferation maybe up to 91.2%. The seedings on subculture medium could normally multiply, root and regrowth.

关键词

流苏石斛/种子/非共生萌发

Key words

Dendrobium fimbriatum Hook. /seed/nonsymbiotic germination

分类

园艺学与植物营养学

引用本文复制引用

余乐,兰芹英,汤庚国..流苏石斛种子非共生萌发的研究[J].福建林学院学报,2011,31(4):346-348,3.

基金项目

中国科学院战略生物资源技术支撑体系专项项目(CZBZX-1). (CZBZX-1)

福建林学院学报

OA北大核心CSCDCSTPCD

2096-0018

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