福建林学院学报2011,Vol.31Issue(4):346-348,3.
流苏石斛种子非共生萌发的研究
Study on nonsymbiotic sproution of Dendrobium fimbriatum Hook.
摘要
Abstract
Seeds of Dendrobium fimbriatum were sown aseptically for protcorms induced and organogenes, also the effects of different temperature, hormones combinations on the seed nonsymbiotic germination were studied. The results showed that protcorms, green and big, were induced after 7 - 15 d on KC basal medium with 2% sucrose, solidified with 0.7% agar at 25 ℃ and the seed germination rate maybe up to 98.3%. The actions of protcorm inducing and seeding differentiation were effective when they were cultured on KC medium +2 mg.L-1 6-BA +0.1 -0.5 mg.L-1 NAA with 3% sucrose , solidified with 0.7% agar at 25 ℃. The percentage of induction and proliferation maybe up to 91.2%. The seedings on subculture medium could normally multiply, root and regrowth.关键词
流苏石斛/种子/非共生萌发Key words
Dendrobium fimbriatum Hook. /seed/nonsymbiotic germination分类
园艺学与植物营养学引用本文复制引用
余乐,兰芹英,汤庚国..流苏石斛种子非共生萌发的研究[J].福建林学院学报,2011,31(4):346-348,3.基金项目
中国科学院战略生物资源技术支撑体系专项项目(CZBZX-1). (CZBZX-1)