安徽农业科学2011,Vol.39Issue(28):17161-17163,3.
孔雀石绿对蚕豆根尖细胞有丝分裂的影响
Effect of Malachite Green on Mitosis of Vicia faba Root Tip Cell
于淑池 1沈燕霞 1池玥兰 1吴霞1
作者信息
- 1. 湖州师范学院生命科学学院,浙江湖州313000
- 折叠
摘要
Abstract
[Objective] The research aimed to study the effect of malachite green (MG) on mitosis of Vicia faba root tip cell. [Method] Dif ferent concentrations of malachite green were used to treat Viciafaba root lip cell for 12.24,36,48 and 72 h.The mitosis of Vieia fa a root tip ceil was observed by conventional chromosome compression technology ,and the mitotic index ( MI) of each treatment group was counted. [ Re sult] As the treated time prolonged,MI presented the M-shaped variation trend,which illustrated that Vicia faba root tip had a adaptive process to MC. As the concentration of MC increased,Ml gradually rote. When the concentration of MG was during 0.50 - 1. 00 mg/L, Ml was the maximum. When the concentration of MG further increased,MI decreased. It showed that MG was favorable for the plant growth in the certain concentration range. [Conclusion] The research could provide the basis for the reasonable utilization of MG,and the reference for evaluating the environmental harm of MG.关键词
孔雀石绿/蚕豆/根尖细胞/有丝分裂Key words
Malachite green/ Vicia faba/Root tip cell/Mitosis分类
农业科技引用本文复制引用
于淑池,沈燕霞,池玥兰,吴霞..孔雀石绿对蚕豆根尖细胞有丝分裂的影响[J].安徽农业科学,2011,39(28):17161-17163,3.