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孔雀石绿对蚕豆根尖细胞有丝分裂的影响

于淑池 沈燕霞 池玥兰 吴霞

安徽农业科学2011,Vol.39Issue(28):17161-17163,3.
安徽农业科学2011,Vol.39Issue(28):17161-17163,3.

孔雀石绿对蚕豆根尖细胞有丝分裂的影响

Effect of Malachite Green on Mitosis of Vicia faba Root Tip Cell

于淑池 1沈燕霞 1池玥兰 1吴霞1

作者信息

  • 1. 湖州师范学院生命科学学院,浙江湖州313000
  • 折叠

摘要

Abstract

[Objective] The research aimed to study the effect of malachite green (MG) on mitosis of Vicia faba root tip cell. [Method] Dif ferent concentrations of malachite green were used to treat Viciafaba root lip cell for 12.24,36,48 and 72 h.The mitosis of Vieia fa a root tip ceil was observed by conventional chromosome compression technology ,and the mitotic index ( MI) of each treatment group was counted. [ Re sult] As the treated time prolonged,MI presented the M-shaped variation trend,which illustrated that Vicia faba root tip had a adaptive process to MC. As the concentration of MC increased,Ml gradually rote. When the concentration of MG was during 0.50 - 1. 00 mg/L, Ml was the maximum. When the concentration of MG further increased,MI decreased. It showed that MG was favorable for the plant growth in the certain concentration range. [Conclusion] The research could provide the basis for the reasonable utilization of MG,and the reference for evaluating the environmental harm of MG.

关键词

孔雀石绿/蚕豆/根尖细胞/有丝分裂

Key words

Malachite green/ Vicia faba/Root tip cell/Mitosis

分类

农业科技

引用本文复制引用

于淑池,沈燕霞,池玥兰,吴霞..孔雀石绿对蚕豆根尖细胞有丝分裂的影响[J].安徽农业科学,2011,39(28):17161-17163,3.

安徽农业科学

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