安徽农业科学2011，Vol.39Issue(22)：13282-13284,3.
紫色链霉菌产褐藻胶裂解酶培养条件的初步研究
Initial Research on the Culture Condition of Alginate Lyase Production by Streptomyces violaceoruber
摘要
Abstract
[Objective] The research aimed to study initially the best culture condition of alginate lyase production by Streptomyces violaceorulier. [ Method] Streptomyces violaceoruber whose alginate lyase activity was high was as the initial strain. The influences of substrate concentration, nitrogen source, culture temperature, culture time, liquid volume, KH2PO4 concentration, MgSO4·7H2O concentration on the enzyme production were inspected. [ Result] The best enzyme production condition: peptone and yeast extract were as the nitrogen source, 1.4% alginate, 0.4% KH2PO4, 0.06% MgSO4·7H2O, the culture temperature was 30 X., and the culture time was 6 d. [Conclusion] The research laid the foundation for the fermentation, enzyme production of Streptomyces violaceoruber and the preparation of alginate lyase.关键词
紫色链霉菌/褐藻胶裂解酶/培养条件Key words
Streptomyces violaceoruber/ Alginate lyase/ Culture condition分类
农业科技引用本文复制引用
刘航,曹海龙,李曙光,杜昱光..紫色链霉菌产褐藻胶裂解酶培养条件的初步研究[J].安徽农业科学,2011,39(22):13282-13284,3.基金项目
国家高技术研究发展计划项目(2011AA090704) （2011AA090704）
中国科学院知识创新工程重要方向项目(KSCX2-YW-G-041) （KSCX2-YW-G-041）
公益性(农业)科研专项(200903052) （农业）
重大新药创制(2009ZX09501-011). （2009ZX09501-011）
