中国输血杂志2011，Vol.24Issue(10)：833-836,4.
全血放置4℃制备24h冰冻血浆的质量研究
Study on the quality of plasma prepared from 4℃ stored whole blood
王甜甜 1赵树铭 1王世春 1夏代全 2黄扑 3王泽荣 1林放 1刘凤君 1黎儒青 1郭辉4
作者信息
- 1. 第三军医大学西南医院输血科中国人民解放军重庆血站,重庆400038
- 2. 重庆市血液中心
- 3. 重庆市黔江中心血站
- 4. 第三军医大学西南医院检验科
- 折叠
摘要
Abstract
Objective To investigate the manufacture procedure and quality of plasma separated from whole blood within 24 hrs of collection. Methods Twenty four bags of 400 ml whole blood (WB,n=24) were collected according to the standard procedures from volunteer donors,and then each bag was divided into two equal units. One unit from each bag was put to group 1 and the other unit to group 2. All units in group 1 were leukodepleted within 6hrs of whole blood collection. Plasma was separated from 12 of group 1 units immediately after leukodepletion (FFP). The other 12 leukodepleted u-nits of group 1 were stored at 4℃ overnight before separation of plasma ( I-FP24). Whole blood units of group 2 were stored overnight at 4℃ and then leukoreduced and plasma separated ( Ⅱ -FP24). All plasma was stored in -30℃. refrigerator before testing. Coagulation factors Fib, F V , FⅦ, and FⅦ, and some biochemical indicators were analyzed. Results The mean levels of Fib,FⅤ,FⅦ,FⅦ in FFP were (2.08 ±0.16) g/L,(86.57 ±18.73)% ,(91.71 ±25.53)% and (97.65 ±25.99)%, respectively, while those values were reduced by 22.6% ,29. 38% ,32. 89% ,and 40.71% respectively for I-FP24, and by 1.9%, -2.09%, 14.91%, and 32.86% respectively for Ⅱ-FP24. The levels of FⅦ in FFP and Ⅱ-FP24 were (97.65 ±25.99)% and (65.56 ± 13.93)% .respectively (P<0.05) , and the levels of FⅤ in Ⅱ-FP24 and Ⅰ-FP24 were (88.38 ±15.97)% and (61. 14 ± 13.76)% .respectively ( P < 0. 05 ). Compared to FFP and Ⅰ-FP24,Ⅱ-FP24 had higher levels of K+ (4. 33 ±0.28 mmol/L),LDH (145.70 ±26. 00mmol/L) and FHb (129.96 ±34.33mg/ L). Conclusion Plasma prepared from WB leukodepleted within 6 hrs, and then stored overnight at A℃ has much lower levels of coagulation factors. FP24 is best prepared from WB stored overnight at 4% and then leukodepleted and plasma separated within 24 hrs.关键词
24h冰冻血浆/冰冻血浆制备/全血放置4℃/过夜/血浆质量/凝血因子Key words
24h frozen plasma/Frozen plasma manufacture procedure/Whole blood stored at 4℃ /Over night/Plasma quality/ Coagulation factors分类
医药卫生引用本文复制引用
王甜甜,赵树铭,王世春,夏代全,黄扑,王泽荣,林放,刘凤君,黎儒青,郭辉..全血放置4℃制备24h冰冻血浆的质量研究[J].中国输血杂志,2011,24(10):833-836,4.
