中国输血杂志2011，Vol.24Issue(10)：850-853,4.
基于CFSE染色的小鼠淋巴细胞体内示踪方法的建立
A tracking method for CFSE-labeled lymphocytes in vivo
摘要
Abstract
Objective To establish a sensitive method for tracking mouse lymphocytes labeled with carbaxyfluorescein diacetate succinimidyl ester (CFSE) in vivo. Methods Mouse lymphocytes were labeled with various concentrations of CFSE in different buffers. Based on fluorescence intensity,the optimal labeling condition was selected. Labeled lymphocytes were transfused into mouse and the distribution and duration of the cells in various organs were measured by flow cytometry. Results The mean fluorescence intensity of CFSE+ cell in PBS groups were 596,793,1 123,1 596, and 1 737 .which were significantly higher than those in RPMI-1640 groups (P <0. 05). The death rates of CFSE+ cells were 8. 7% ,8. 4% , 8. 6% ,and 9.0% (P>0.05) at different concentrations of CFSE in RPMI-1640 groups,increasing along with the increasing concentrations of CFSE in PBS groups ( P < 0.05 ). The CFSE-labeled lymphocytes can be detected for as long as two months in many organs after transfusion. Conclusion Under optimal conditions, CFSE-labeling is reliable,stable and convenient for lymphocyte tracing in vivo.关键词
CFSE/淋巴细胞/流武细胞术/细胞标记Key words
Key words/ CFSE /Lymphocyte/ Flow cytometry/ Cell labeling分类
基础医学引用本文复制引用
汤永永,王宇玲,唐颖,张玉华,段文元,赵敬湘,魏广智,张秀媛,李鹏飞,穆思媛..基于CFSE染色的小鼠淋巴细胞体内示踪方法的建立[J].中国输血杂志,2011,24(10):850-853,4.基金项目
全军医药卫生科研基金(09MA023) （09MA023）
