福建农业学报2011,Vol.26Issue(5):774-777,4.
激素水平对福建道地瓜蒌组培快繁的影响
Effect of Hormone on Tissue Culture and Rapid Propagation of Trichosanthes kirilowii Maxim
摘要
Abstract
Effect of hormone application on tissue culture of Trichosanthes kirilcrwii Maxim was investigated. Stems with axillary buds were used as explantlet, and MS for the basic medium. A vailable methods for the rapid propagation to clone T. Kirilowii Maxim are reviewed. In the experiment, the young adventitious buds continuously appeared for 15 days in the culture medium of MS + 1. 0 mg · L-1 BA. In 30 days, the average number of shoots was 6. 5, and the average shoot ratio was 81. 25%. The excised plantlets could produce root 15 days after inoculation on the MS + 0. 4 mg NAA L-1 medium. They continued to gradually develop into normal plants. A single plant had an average of 5 roots. The average rooting percentage was 100%. This rapid propagation method for T. Kirilowii Maxim appeared to offer many benefits at low cost.关键词
福建/瓜萎/组织培养/激素水平Key words
Fujian/Trichosanthes kirilo-wii Maxim/tissue culture/hormone application分类
农业科技引用本文复制引用
郑向丽,叶花兰,徐国忠,陈敏健..激素水平对福建道地瓜蒌组培快繁的影响[J].福建农业学报,2011,26(5):774-777,4.基金项目
福建省农业科学院科技创新团队建设基金项目(STIF-Y01) (STIF-Y01)
福建省农业科学院“双百行动”计划项目(sbmb1104-1) (sbmb1104-1)
福州市科技计划项目(2011-S-76) (2011-S-76)