福建农业学报2011,Vol.26Issue(5):778-782,5.
短葶山麦冬茎尖培养快速繁殖技术研究
Micropropagation for Liriope muscari (Desne.) Bailey
摘要
Abstract
Shoot tips from Liriope muscari (Desne. ) Bailey were used as explants to grow on media with varied sterilization time, under induced differentiation, as well as different strengthening and rooting treatments, and transplantation conditions. The shoot tips were cut from the plants and cultured on MS medium containing 2. 0 mg · L-1 BA and 0. 2 mg · L-1 NAA. The highest differentiating rate of 90% was achieved. The medium with 4. 0 mg · L-1BA and 0. 2 mg · L-1 NAA was suitable for multiple-shoot proliferation. A certain concentrations of BA promoted the shoot differentiation. The optimized medium for seeding and rooting was when MS was mixed with 0. 5 mg · L-1 IBA, which improved the root coefficient to 6. 87. Peat soil was found to be the best medium material for the transplantation.关键词
短葶山麦冬/茎尖培养/快速繁殖Key words
Liriope muscari (Desne.) Baileys/shoot tip culture/miacropropagation分类
农业科技引用本文复制引用
万学锋,赖钟雄,陈菁瑛..短葶山麦冬茎尖培养快速繁殖技术研究[J].福建农业学报,2011,26(5):778-782,5.基金项目
福建省科技平台建设项目(2008Y2003) (2008Y2003)
国家“十一五”科技支撑计划(2006BAI06A11-03) (2006BAI06A11-03)