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短葶山麦冬茎尖培养快速繁殖技术研究

万学锋 赖钟雄 陈菁瑛

福建农业学报2011,Vol.26Issue(5):778-782,5.
福建农业学报2011,Vol.26Issue(5):778-782,5.

短葶山麦冬茎尖培养快速繁殖技术研究

Micropropagation for Liriope muscari (Desne.) Bailey

万学锋 1赖钟雄 2陈菁瑛3

作者信息

  • 1. 福建省农业科学院农业生物资源研究所,福建福州350003
  • 2. 福建省农业科学院药用植物研究中心,福建福州 350003
  • 3. 福建农林大学园艺植物生物工程研究所,福建福州350002
  • 折叠

摘要

Abstract

Shoot tips from Liriope muscari (Desne. ) Bailey were used as explants to grow on media with varied sterilization time, under induced differentiation, as well as different strengthening and rooting treatments, and transplantation conditions. The shoot tips were cut from the plants and cultured on MS medium containing 2. 0 mg · L-1 BA and 0. 2 mg · L-1 NAA. The highest differentiating rate of 90% was achieved. The medium with 4. 0 mg · L-1BA and 0. 2 mg · L-1 NAA was suitable for multiple-shoot proliferation. A certain concentrations of BA promoted the shoot differentiation. The optimized medium for seeding and rooting was when MS was mixed with 0. 5 mg · L-1 IBA, which improved the root coefficient to 6. 87. Peat soil was found to be the best medium material for the transplantation.

关键词

短葶山麦冬/茎尖培养/快速繁殖

Key words

Liriope muscari (Desne.) Baileys/shoot tip culture/miacropropagation

分类

农业科技

引用本文复制引用

万学锋,赖钟雄,陈菁瑛..短葶山麦冬茎尖培养快速繁殖技术研究[J].福建农业学报,2011,26(5):778-782,5.

基金项目

福建省科技平台建设项目(2008Y2003) (2008Y2003)

国家“十一五”科技支撑计划(2006BAI06A11-03) (2006BAI06A11-03)

福建农业学报

OACSTPCD

1008-0384

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