安徽农业科学2011，Vol.39Issue(32)：19798-19799,19838,3.
蛇足石杉中石杉碱甲的酶法提取研究
Study on the Extraction of Huperzine A from Huperzia serrata by Enzymatic Method
摘要
Abstract
[Objective] To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method. [ Method] U-sing cellulase to hydrolyze the cytoderm, orthogonal test was carried out to optimize the extraction technology of huperzine A from Huperaa serrata by enzymatic method. [ Result] The optimum extraction technology of huperzine A from Huperaa serrata by enzymatic method was a combination of 0.125 g cellulase,4.5 pH,60 ℃ enzymolysis temperature and 2.5 h enzymolysis time,under which the extraction rate of huperzine A could reach 0.589%.. Compared with the acidsoak method, the extraction rate of huperzine A by enzymatic method increased by 40.3%. Besides , hydrolysis temperature was the biggest influence factor to the extraction of huperzine A from Huperaa serrata by enzymatic method. [ Conclusion] This method was simple,convenient and efficient,which could be used for extracting huperzine A from Huperzia serrata.关键词
蛇足石杉(Huperzia serrata(Thunb. ex Murray)Trev.)/石杉碱甲(Huperzlne A)/酶法提取/纤维素酶Key words
Huperzia serrata/Huperzine A/Enzymatic extraction/Cellulase分类
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易学文,张利,罗容珍,杜平..蛇足石杉中石杉碱甲的酶法提取研究[J].安徽农业科学,2011,39(32):19798-19799,19838,3.
