化学工程2011，Vol.39Issue(10)：6-10,5.
溶氧探测补料技术发酵生产类人胶原蛋白的研究
Fed-batch production of human-like collagen with recombinant Escherichia coil using feed-up DO-transient control
摘要
Abstract
To control the acetate production and achieve a high-level expression of the target protein in the different-scale bioreactors during fed-batch production of human-like collagen (HLC) with recombinant Escherichia coli. A probing feeding strategy using feed-up DO-transient control was employed in two different-scale bioreactors and the dissolved oxygen response signal was used to detect the acetate production in pulsed fed-batch cultivation of E. Coli. The final dry cell weight (DCW) of 69.1 g/L and HLC mass concentration of 13.1 g/L were obtained on the laboratory scale and the results are similar to that optimized in the previous study. When the process was scaled up to pilot-scale (500 L) , the final DCW decreased from 80.3 g/L to 54.1 g/L with non-induced cultivations while acetate mass concentration was similar on the both scales. The difference in DCW was mainly resulted from the difference in oxygen transfer capacity. The final DCW of 51.7 g/L and HLC mass concentration of 9.6 g/L were obtained on the pilot-scale upon optimization of induction timing, and the results are satisfied based on the HLC content. The feed-up DO-transient control strategy can be successfully employed to control the acetate production and achieve a high-level expression of the target protein during the fed-batch production of HLC with recombinant E. Coli.关键词
溶氧探测补料技术/乙酸/重组大肠杆菌/类人胶原蛋白Key words
feed-up DO-transient control/acetate/recombinant Escherichia coli/human-like collagen分类
化学化工引用本文复制引用
薛文娇,范代娣..溶氧探测补料技术发酵生产类人胶原蛋白的研究[J].化学工程,2011,39(10):6-10,5.基金项目
国家自然科学基金资助项目(31000019) （31000019）
