福建师范大学学报（自然科学版）2011，Vol.27Issue(6)：77-82,6.
苏云金芽孢杆菌aiiA基因启动子的克隆及功能分析
Cloning and Functional Verification of Promoter of aiiA Gene from Bacillus thuringiensis
摘要
Abstract
A pair of specific PCR primers were designed according to the aiiA gene (AY46O124) reported. The aiiA promoter sequence was successfully amplified. The GFP function verification vector was constructed by replacing the T7 promoter in the expression vector pET-28a-GFP with aiiA gene promoter sequence. Sequence alignment and bioinfor matics software analysis found that the sequence contains six possible promoter regions and nineteen distinguished types of transcriptional binding sites. Bacterial liquid could emit fluo rescence observed by the fluorescence microscope. The results indicated that the aiiA gene promoter had the promoter activity.关键词
苏云金芽胞杆菌/启动子/绿色荧光蛋白/功能鉴定Key words
Bacillus thuringiensis/ promoter/ green fluorescent protein/ function verification分类
农业科技引用本文复制引用
温真,杨彩云,林丽玉,毛惠民,杨梅..苏云金芽孢杆菌aiiA基因启动子的克隆及功能分析[J].福建师范大学学报（自然科学版）,2011,27(6):77-82,6.基金项目
福建省科技厅资助项目(2009N0032) （2009N0032）
福建省教育厅资助项目(JA08041) （JA08041）
