中山大学学报（医学科学版）2011，Vol.32Issue(6)：708-712,5.
骨髓间充质干细胞在致敏小鼠体内的示踪归巢实验
Bone Marrow Mesenchymal Stem Cells Homing in Sensitized Mice
摘要
Abstract
[Objective] To explore the homing characteristics of bone marrow mesenchymal stem cells (MSCs) in the allogeneic splenocytes sensitized mice. [Method] After normal allogene bone marrow MSCs were cultured and evaluated, MSCs were labeled by 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) in vitro. After injected CFSE labeled MSCs (CFSE + cells) through vena caudalis in 6,24,and 48 h,homing trace were explored in the different organs both in the sensitized and normal mice group (each group 10 samples mice) through frozen section and flow cytometry (FCM) detection methods. [Results] Both in the methods of frozen section and FCM, we discovered in the sensitized mice group MSCs were aggregated and homing in spleen and liver quickly and lastingly, while in the normal mice group MSCs were homing to the bone marrow in the end. Among those FCM quantitative result, in 6 h the percentage of spleen CFSE + cells in the sensitized mice group was (3.78±0.66)%,significant difference was found in spleen between the sensitized and normal group at the same time (P <0.05); while in 48 h, the percentage of bone marrow CFSE+ cells in the sensitized and normal mice group were (0.43±0.35)% and (5.40±1.58)% , respectively (P< 0.05). [Conclusion] Immunity organs such as liver and spleen, influenced by the sensitized of allogeneic splenocyted transfusion, had the orientating function in the normal allogene bone marrow MSCs homing, which interrupted the homing to the bone marrow in the normal condition.关键词
致敏小鼠/骨髓间充质干细胞/归巢示踪Key words
sensitized mice/ mesenchymal stem cells/ homing trace分类
医药卫生引用本文复制引用
洪冬玲,吴燕峰,许吕宏,方建培..骨髓间充质干细胞在致敏小鼠体内的示踪归巢实验[J].中山大学学报（医学科学版）,2011,32(6):708-712,5.基金项目
国家自然科学基金(30872383) （30872383）
