中山大学学报（医学科学版）2011，Vol.32Issue(6)：741-746,6.
硫化氢通过抑制iNOS-NO通路对抗化学性缺氧引起的PC12细胞损伤
Hydrogen Sulfide Protects PC12 Cells against Chemical Hypoxia-induced Injury by Inhibiting iNOS-NO Pathway
摘要
Abstract
[Objective] To investigate whether hydrogen sulfide (H2S) protected PC 12 cells against chemical hypoxia-induced injury by inhibiting iNOS-NO pathway.[Method] PC12 cells were treated with cobalt chloride (CoCl2) to set up a chemical hypoxia-induced cellular injury model. Cell viability was tested by Cell Counter Kit (CCK-8); morphological changes of apoptotic cells were detected by Hoechst33258 staining; Apoptotic rate was evaluated by propidium iodide staining and flow cytometry (FCM) ; Nitrite accumulation, an indicator of nitrogen monoxidum (NO) production, was measured in cell culture supernatants using the Griess reagent; the expression of the inducible enzyme of NO (iNOS) was determined by Western blot assay. [Results]Exposure of PC 12 cells to 600 u.mol/L CoCl2 for 24 h significantly enhanced iNOS expression. Pretreatment with 400 |xmol/LNaHS (a donor of H2S) for 30 min prior to exposure of PC 12 cells to 600 (jumol/L CoCl2 not only inhibited CoCl2-induced increase in expression of iNOS and NO production, but also protected PC 12 cells against injuries induced by 600 Jjimol/L CoCl2, enhancing cell viability and decreasing amount of apoptotic cells. Similarly, pretreatment with L-Canavanine (10 u,mol/L) , an inhibitor of iNOS for 60 min prior to exposureof PC 12 cells to CoCl2 also conferred the same cytoprotective effect of H2S. In addition, pretreatment with SB203580, an inhibitor of p38MAPK, for 60 min prior exposure of PC 12 cells to 600 (Jimol/L C0CI2 could also down-regulate the expression of iNOS induced by CoCl2. [Conclussions] The iNOS-NO pathway mediates CoCl2-induced injury and H^S can protect PC 12 cells against chemical hypoxia-induced injury by inhibiting iNOS-NO pathway.关键词
硫化氢/氯化钴/诱导型一氧化氮合酶/一氧化氮/刀豆氨酸/凋亡Key words
hydrogen sulfide/ cobalt chloride/ iNOS/ NO/ L-canavine/ apoptosis分类
生物科学引用本文复制引用
郑东诞,兰爱平,莫利求,杨战利,杨春涛,王秀玉,郭润民,陈培熹,冯鉴强..硫化氢通过抑制iNOS-NO通路对抗化学性缺氧引起的PC12细胞损伤[J].中山大学学报（医学科学版）,2011,32(6):741-746,6.基金项目
广东省科技计划项(2010B080701035,2008B080703053) （2010B080701035,2008B080703053）
