安徽农业科学2011，Vol.39Issue(28)：17158-17160,3.
水稻线粒体nad1基因超表达载体的构建和遗传转化
Construction and Genetic Transformation of Over-Expression Vector of nad1 Gene in Rice
摘要
Abstract
[Objective] The aim was to clone the mitochondria related gene nadl and obtain transgenic rice plants with nadl. [ Method] The total RNA was extracted from rice seedlings and reverse transcriped to cDNA. The target gene nadl was amplified with the cDNA as template.Nadl and Rf1b,a sequence of signal peptide of mitochondria,were linked to binary expression vector pCAM8IA130S. 1. The recombinant plasmid was transformed into the callus by Agrobacterium-mediated approach. [ Result] The target gene nadl was 978 bp. The binary expression vector carried nadl and signal peptide of mitochondria was constructed successfully. A lot of transgenic plants were obtained. [ Conclusion] The study will provide basis to investigate the effect of over-expression of nadl on rice plant growth.关键词
水稻/nad1/遗传转化Key words
Rice/nad1/Genetic transformation分类
农业科技引用本文复制引用
李炜,谭艳平,王春台,刘学群..水稻线粒体nad1基因超表达载体的构建和遗传转化[J].安徽农业科学,2011,39(28):17158-17160,3.基金项目
中南民族大学自然基金重点项目(Y2Z08010). （Y2Z08010）
