安徽农业科学2011，Vol.39Issue(29)：17920-17922,3.
肉牛肌生成抑制素成熟蛋白编码序列的克隆与原核表达
Cloning and Prokaryotic Expression of the Coding Sequence of Maturation Protein of Bovine Myostatin Gene
左明坤 1沈冰蕾 1倪洪波1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆163319
- 折叠
摘要
Abstract
[Objective] The aim was to test and verify maturation protein of bovine myostatin(MSTN) gene based on prokaryotic expression,to provide basis for inoculation experiment of mice. [ Method] RT-PCR method was used to amplify coding sequence of maturation protein of bovine myostatin(MSTN) gene, then after double digestion, amplification products were cloned to expression vector pET28a( + ), transform escherichia coli BL21, after induction of IPTG, SDS-PAGE and Western-blot were used to detect expression of results of recombinant protein.[Result] Anti-His-Tag identification was correct. [Conclusion] Beef cattle MSTN were expressed in prokaryotic expression system correctly.关键词
肉牛/肌生成抑制素/克隆/原核表达/生物活性Key words
Beef cattle/ Mysotatin/ Cloning/ Prokaryotic expression/ Bioactivity分类
农业科技引用本文复制引用
左明坤,沈冰蕾,倪洪波..肉牛肌生成抑制素成熟蛋白编码序列的克隆与原核表达[J].安徽农业科学,2011,39(29):17920-17922,3.
