安徽农业科学2011，Vol.39Issue(23)：14162-14163,14169,3.
HPLC法测定喙尾琵琶甲药材中氨基酸的含量
Determination of Amino Acids in Blaps Rynchopetera Fairmaire by HPLC
摘要
Abstract
[Objective ] The aim was to establish an HPLC method for determining amino acids in Blaps rynchopetera Fairmaire. [ Method ] After being hydrolyzed with HCl and derivating in precolumn with o-phthalaldehyde and β-mercaptoethanol, the amino acids were eluted by gradient e-lution and were detected with an UV detector (detection wavelength:338 nm). The mobile phase consisted of 0. 55% NaH2PO4( pH 7. 80) -methanol-acetonitrile,and column temperature was set at 35 ℃. [ Result]The derivatives of amino acids with o-phthalaldehyde could be separated with a stable base line within 40 min. The calibration curves for amino acids had good linearity(r =0. 997 0-1. 000 0,n =6). The average recoveries were 90.16% - 104.1% ,and RSDs were 1.89% -5.92%. [Conclusion]The method was simple,accurate and well reproduced for the determination of amino acids in Blaps rynchopetera Fairmaire.关键词
喙尾琵琶甲/氨基酸/柱前衍生化/高效液相色谱Key words
Blaps rynchopetera Fairmaire/Amino acids/Pre-column derivation/HPLC分类
生物科学引用本文复制引用
肖培云,杨永寿,施贵荣,李龙星..HPLC法测定喙尾琵琶甲药材中氨基酸的含量[J].安徽农业科学,2011,39(23):14162-14163,14169,3.基金项目
国家自然科学基金资助项目(81060329). （81060329）
