色谱2011，Vol.29Issue(10)：967-973,7.DOI:10.3724/SP.J.1123.2011.00967
液相色谱-串联质谱法测定肉及肉制品中利谷隆及其代谢产物3,4-二氯苯胺残留
Determination of linuron and its metabolite 3,4-dichloroaniline residues in meat and meat products using liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of linuron and its metabolite 3,4-dichloroaniline residues in pork, liver, kidney, casings, canned steam pork and sausage. The sample was extracted with a mixture of acetone-acetonitrile (5:95, v/v). Most of the lipids in the extract were eliminated by freezing-lipid filtration. After Florisil solid phase extraction cleanup, the linuron and its metabolite residues were determined by LC-MS/MS, and quantified by internal standard method. In the range of 1 -500 μg/L, both linuron and 3,4-dichloroaniline showed good linearity with the correlation coefficients (r) more than 0. 998. The limit of quantification (S/N > 10) was 10 μg/kg, and the limit of detection (S/N > 3) was 5 μg/kg for each analyte. The recoveries of linuron and 3 ,4-dichloroaniline in meat and meat products at three spiked levels were in the ranges of 88. 3% -101.2% and 91. 6% - 101.6%, and the relative standard deviations were in the ranges of 4. 8% - 13. 7% and 4. 7% - 11. 8%, respectively. The results demonstrated that the proposed method can meet the requirements for the determination of Unuron and 3,4-dichloroaniline in meat and meat products.关键词
液相色谱-串联质谱/利谷隆/3,4-二氯苯胺/肉/肉制品Key words
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/ Unuron/ 3,4-dichloroaniline/ meat/ meat products分类
化学化工引用本文复制引用
郭德华,伊雄海,曲栗..液相色谱-串联质谱法测定肉及肉制品中利谷隆及其代谢产物3,4-二氯苯胺残留[J].色谱,2011,29(10):967-973,7.基金项目
上海技术标准专项基金项目(10DZ050410)、上海技术贸易措施应对专项基金项目(09TBT006)和国家质量监督检验检疫总局项目(2011IK232) （10DZ050410）
