渔业科学进展2011，Vol.32Issue(5)：44-50,7.
脊尾白虾热休克蛋白HSP90基因的原核表达与鉴定
High level prokaryotic expression and identification of heat shock protein 90 in Exopalaemon carinicauda
摘要
Abstract
The aim of this study was to set up a high level prokaryotic expression of heat shock protein 90(HSP90) of Exopalaemon carinicauda in E. Coli Rosetta. The HSP90 gene of E. Carinicauda was cloned into prokaryotic expression vector pET-30a, which was confirmed by double-endonuclease digestion and DNA sequencing. The recombinant vector was transformed into E. Coli Rosetta and was induced to express under different temperatures, durations, IPTG concentrations and OD600. The expressed product was identified by SDS-PAGE and an 82. 7 kD protein (determined by mass spectrometry) was found. The expression level varied under different conditions. The optimal expression was achieved under induction conditions of 1 mmol/L IPTG,37℃ , OD600 = 0. 58, and 7 h.关键词
脊尾白虾/热休克蛋白90/原核表达Key words
Exopalaemon carinicauda/Heat shock protein 90 (HSP90)Prokaryotic expression分类
农业科技引用本文复制引用
韩俊英,李健,李吉涛,陈萍,李华..脊尾白虾热休克蛋白HSP90基因的原核表达与鉴定[J].渔业科学进展,2011,32(5):44-50,7.基金项目
公益性行业(农业)科研专项(nyhyzx07-042,200803012) （农业）
国家虾产业技术体系(CARS-47)和科技部农业科技成果转化资金项目(2010GB23260589)共同资助 （CARS-47）
