武警医学2011,Vol.22Issue(11):930-931,2.
紫外分光光度法测定参七复脉片中总皂苷含量
Determination of total triterpenoid saponin Shenqifumai tablets by UV spectrophotometry
摘要
Abstract
Objective To establish an ultraviolet spectrophotomelric determination method for total triterpenoid saponin in Shenqifumai tablets. Methods The content was determined with 5% vanillin - glacial acetic acid - sulphuric acid - perchloric acid mixture as the color developing reagent and ginsenoside Re as the standard sample. Results Ginsenoside Re had the linear range of 3. 04 - 15. 2μg( r = 0. 9993). The average recovery was 97. 64% ( RSD = 0. 65% ). Conclusions This method is reliable , accurate and specific. It can be used for quality control of Shenqifumai tablets.关键词
参七复脉片/总皂苷/紫外分光光度法/含量测定Key words
shenqifumai tablets/ total saponin/ UV spectropholometrg/ content detennination分类
中医学引用本文复制引用
刘凯,陈江,肖引,彭国华,李广琪,陈芳,李勃..紫外分光光度法测定参七复脉片中总皂苷含量[J].武警医学,2011,22(11):930-931,2.基金项目
武警部队后勤部课题资助项目(编号wJ HQ2008-05) (编号wJ HQ2008-05)