武警医学2011，Vol.22Issue(11)：938-942,5.
高压氧预处理对高原肺水肿大鼠肺水通道蛋白-5的影响
Effect of hyperbaric oxygen preconditioning on expression of lung aquaporin - 5 in rat models of high altitude pulmonary edema
李茁 1赵立明 1王丛 1刘雪华 1吕春凤 2高春锦1
作者信息
- 1. 100020,首都医科大学附属北京朝阳医院高压氧科
- 2. 100076,北京航天总医院
- 折叠
摘要
Abstract
Objective To investigate ihe possibility of applying hyperbaric oxygen preconditioning { HBO - PC) to reduce or prevent high - altitude pulmonary edema ( HAPE) and to find out if aquaporin -5 ( AQP5) is involved in HAPE pathogenesis using a rat model. Methods Rats were divided into three groups: control, HBO and HAPE. Western blotting and real-time PCR were used to analyze (he expression of AQP5 in the lung. The wet -to - dry weighl ratio ( W/D weight ratio) and morphology of the lung were also examined. Results An increased lung W/D weight ratio was demonstrated in the HAPE group compared with the control group. Marked interstitial edema in all ling sections in the HAPE group, and small changes in 10 of the 13 sections were present in the HBO group. Lung-injury scores in the HBO group were much lower than those in the HAPE group. AQP5 in the HAPE group decreased significantly at the protein and gene level, compared with those in the control group. Protein and mRNA expression of AQP5 in the HBO group were much higher than those in the HAPE group. Lung injury in the HAPE rats was related positively to the AQP5 expression in the lung. Conclusions HBO - PC can alleviate lung injury in rats caused hy high - altitude hypobaric hypoxia and reduce HAPE incidence. HAPE in rats is associated with a downregulation of expression of AQP5 in the lung. This downregulation can be attenuated by HBO - PC. This study is the first report to introduce HBO - PC in ihe prevention of HAPE and the first investigation of the expression of AQP5 in a rat model of HAPE.关键词
高原肺水肿/高压氧预处理/水通道蛋白5/肺组织病理/肺湿/干比值Key words
high - altitude pulmonary edema/hyperbaric oxygen preconditioning/ Aquaporin 5/lung wet - to - dry weighl ratio/ lung morphology分类
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李茁,赵立明,王丛,刘雪华,吕春凤,高春锦..高压氧预处理对高原肺水肿大鼠肺水通道蛋白-5的影响[J].武警医学,2011,22(11):938-942,5.
