草业学报2011，Vol.20Issue(5)：93-101,9.
过量施氮对嵌合体‘金旗’玉簪叶色、氮代谢关键酶活性及叶绿体超微结构的影响
Effects of excessive application of nitrogen fertilizer on leaf color, key enzymes activities of nitrogen metabolism and chloroplast ultrastructure of a chimera Hosta ‘Gold Standard'
摘要
Abstract
Leaf colors, key enzymes of nitrogen metabolism, and chloroplast ultrastructure were studied in ex cessive application of nitrogen fertilizer cultivation on chimera Hosta 'Gold standard'. The results showed that: chlorophyll content significantly increased or decreased in yellow or green leaf area of the chimera in the 2 g/kg of urea fertilizer treatment respectively, and leaf of chimera completely turned green within 28 d. Total N content, activities of nitrate reductase (NR) and glutamate dehydrogenase (GDH) of chimera leaf reached to their highest level after 7 days treatment; activities of glutamine synthetase (GS) reached to their highest level after 14 days treatment, and the changing trends of glutamate synthetase (GOGAT) activities was similar to chlorophyll content on chimera leaf. The develepoment of chloroplast ultrastructure, which lacks normal grana stacking, and contains large number of vacuoles was severely inhibited in the yellow leaf area of the chemera. After 28 d fertilization treatments, normal thylakoid lamellar structure was formed in chloroplast of yellow leaf area, which was similar to the chloroplast structure of the green leaf area and leaf of non-chimera Hosta.关键词
嵌合体/‘金旗’玉簪/氮肥/叶色/氮代谢/叶绿体超微结构Key words
chimera/ Hosta 'Gold standard'/ nitrogen fertilizer/ leaf color/ nitrogen metabolism/ chloroplast ultrastructure分类
农业科技引用本文复制引用
张金政,刘岳路,李晓东,刘洪章,孙国峰,何卿..过量施氮对嵌合体‘金旗’玉簪叶色、氮代谢关键酶活性及叶绿体超微结构的影响[J].草业学报,2011,20(5):93-101,9.基金项目
中国科学院知识创新重要方向性项目(KSCX2-EW-B-2,KSCX2-EW-B-5)资助. （KSCX2-EW-B-2,KSCX2-EW-B-5）
