中国药房2011，Vol.22Issue(45)：4268-4270,3.
阳离子脂质/DNA复合物气雾剂的制备及其体外细胞转染研究
Preparation of Aerosols Containing Cationic Lipid/DNA Complexes and in Vitro Cell Transfection Study
摘要
Abstract
OBJECTIVE: To prepare Aerosols containing DOTAP/DNA complexes, and to study their cell transfection efficiencies in vitro. METHODS: DOTAP/DNA complexes were prepared by modified Bligh and Dyer extraction method, which could be scattered in ether evenly with different N/P ratios. Drug-loading rate and particle size distributions were examined by inorganic phosphate assay and laser granularity distributing apparatus. Aerosols containing DOTAP/DNA complexes were further filled. The cell transfection efficiencies and cytotoxicity of aerosols were determined using human lung adenocarcinoma cell line H1299 as a model. RESULTS: The drug-loading rate of DOTAP/DNA complexes with optimal N/P ratio (2∶1) reached (72 ± 1.580)% , and the particle size was about (188.700 ± 42.770) nm. The cell survival rate after the aerosol application was (91.643 ± 6.970)% , and fluorescence intensity was 1.080×105 RLU·mg-1. CONCLUSIONS: Developed novel Aerosols containing DOTAP/DNA complexes have low toxicity and high cell transfection efficiencies, which may have the potential to be an effective gene delivery formulation to the respiratory tract.关键词
阳离子脂质/DNA复合物/气雾剂/制备/细胞毒性/细胞转染效率Key words
Cationic lipid/DNA complexes/ Aerosol/ Preparation/ Cytotoxicity/ Cell transfection efficiency分类
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盛芝娜,潘怡娜,魏晓慧,徐宇虹..阳离子脂质/DNA复合物气雾剂的制备及其体外细胞转染研究[J].中国药房,2011,22(45):4268-4270,3.
