河南农业大学学报2011,Vol.45Issue(5):568-570,584,4.
猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响
Study on influence of transcriptional profiles of interleukin-18 in Marc-145 cell cultures following infection with porcine parvovirus
摘要
Abstract
In order to survey the host inflammatory responses, in particular interleukin-18 responses of Marc-145 infected with porcine parvovirus (PPV) , and to study the host-PPV interaction, PPV was to used infect the Marc-145 cells to measure and analyze the viral DNA using real-time PCR. The transcript level of interleukin-18 was detected by real-time PCR too. The results showed that the transcript level of interleukin-18 had no obvious change 1 h after inoculation, increased 3 and 24 h after inoculation , and was slightly lower than that of the control 48 h after inoculation. The results indicate infection PPV can upregulate the secretion of interleukin-18 in Marc-145 cell.关键词
猪细小病毒/Marc-145细胞/白细胞介素18/转录时相Key words
porcine parvovirus/Marc-145 cell/IL-18/transcriptional profile分类
农业科技引用本文复制引用
党玉丽,哈斯通拉嘎,耿静微,王瑞宁,郭东辉,胡清林,魏战勇..猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响[J].河南农业大学学报,2011,45(5):568-570,584,4.基金项目
河南省重点科技攻关项目(08210213007) (08210213007)