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猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响

党玉丽 哈斯通拉嘎 耿静微 王瑞宁 郭东辉 胡清林 魏战勇

河南农业大学学报2011,Vol.45Issue(5):568-570,584,4.
河南农业大学学报2011,Vol.45Issue(5):568-570,584,4.

猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响

Study on influence of transcriptional profiles of interleukin-18 in Marc-145 cell cultures following infection with porcine parvovirus

党玉丽 1哈斯通拉嘎 2耿静微 3王瑞宁 3郭东辉 3胡清林 3魏战勇3

作者信息

  • 1. 河南农业大学理学院,河南郑州450002
  • 2. 郑州牧业工程高等专科学校,河南郑州450011
  • 3. 河南农业大学牧医工程学院,河南郑州450002
  • 折叠

摘要

Abstract

In order to survey the host inflammatory responses, in particular interleukin-18 responses of Marc-145 infected with porcine parvovirus (PPV) , and to study the host-PPV interaction, PPV was to used infect the Marc-145 cells to measure and analyze the viral DNA using real-time PCR. The transcript level of interleukin-18 was detected by real-time PCR too. The results showed that the transcript level of interleukin-18 had no obvious change 1 h after inoculation, increased 3 and 24 h after inoculation , and was slightly lower than that of the control 48 h after inoculation. The results indicate infection PPV can upregulate the secretion of interleukin-18 in Marc-145 cell.

关键词

猪细小病毒/Marc-145细胞/白细胞介素18/转录时相

Key words

porcine parvovirus/Marc-145 cell/IL-18/transcriptional profile

分类

农业科技

引用本文复制引用

党玉丽,哈斯通拉嘎,耿静微,王瑞宁,郭东辉,胡清林,魏战勇..猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响[J].河南农业大学学报,2011,45(5):568-570,584,4.

基金项目

河南省重点科技攻关项目(08210213007) (08210213007)

河南农业大学学报

OA北大核心CSCDCSTPCD

1000-2340

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