河南农业大学学报2011，Vol.45Issue(5)：577-580,4.
木质素降解酶对纤维素酶水解糖化的抑制作用
Inhibitory effects of ligninolytic enzymes on cellulase hydrolysis saccharification
摘要
Abstract
The result of pretreatment on corn straw by ligninolytic enzymes produced by white-rot fungi is that hydrolysis saccharification of corn straw is inhibited by ligninolytic enzymes. The lower rate of reducing sugar and glucose concentration are 40. 3% and 2.7% after pretreatment by coarse peroxi-dase fermentation broth; 3.8% and 2.9% after pretreatment by coarse laccase fermentation broth and the lower rate of reducing sugar is 27% after pretreatment by mixed enzyme fermentation broth. This shows that the influence of coarse peroxidase is the greatest, mixing enzymes is secondly and laccase is the lightest. The inhibition was verified by the Novozymes laccase experiments. The experiments of No-vozymes laccase show the lower rate of reducing sugar is 27.5% after pretreatment 24 h by Novozymes laccase and 15. 8% after the viability of Novozymes laccase was inactivated.关键词
白腐真菌/木质素降解酶/纤维素酶/水解糖化Key words
white-rot fungi/ligninolytic enzymes/cellulase/hydrolysis saccharification分类
生物科学引用本文复制引用
冯冲,陈伟,杜风光,宋安东..木质素降解酶对纤维素酶水解糖化的抑制作用[J].河南农业大学学报,2011,45(5):577-580,4.基金项目
阿南省省院科技合作专项资金项目(102106000018) （102106000018）
河南省科技攻关重点项目(092102210110) （092102210110）
