中医药信息2011，Vol.28Issue(6)：14-17,4.
阿霉素-槲皮素复方脂质体的制备
Preparation of Doxorubicin- Quercetin Compound Liposome
摘要
Abstract
Objective: To study the preparation method of doxorubicin - quercetin compound liposome. Method: Liposomes were made of cholesterol (CH) and hydrogenated soybean phosphatidylcholine (HSPC) by film -ultrasound technique and drug - loaded by ammonium sulfate transmembrane gradients, then single factor explorations of drug - lipid ratio, concentration of ammonium sulphate and entrap - temperature were adopted to screen the optimal conditions. The particle size was detected by Zetasizer Nano, while the encapsulation efficiencies of doxorubicin ( DOX) and quercetin (QUE ) were simultaneously determined by RP - HPLC after the free drugs were separated by ultracentrifugal method. Results; The encapsulation efficiencies of doxorubicin and quercetin and the particle size of the liposome with the optimal preparation conditions were as follows; QUE: HSPC = 1: 20mol/mol, DOX: HSPC = 1:7. 9w/w, ( NH4)2SO4 0. 15mol/L, entrap - temperature 55℃; The entrapment efficiencies of DOX, QUE of compound liposome were (90.6 ±0.61)%,(83.3 ±0. 24)% (n =3) and the particle size was 138. 5nm. Conclusion: The optimal preparation of doxorubicin - quercetin compound liposome in this study is stable and feasible.关键词
阿霉素/槲皮素/复方脂质体/硫酸铵梯度法/超速离心Key words
Doxorubicin/ Quercetin/ Compound Liposome/ Ammonium Sulfate Transmembrane Gradients/ Ultracentrifugation分类
医药卫生引用本文复制引用
陈浩,王玉蓉,孙毅坤,周洪伟,黄秀荣,戴俊东..阿霉素-槲皮素复方脂质体的制备[J].中医药信息,2011,28(6):14-17,4.基金项目
国家自然科学基金课题(30801549/H2806) （30801549/H2806）
