河南农业大学学报2011，Vol.45Issue(5)：552-557,6.
棉铃虫酰基辅酶A△9去饱和酶基因的分子鉴定
Molecular identification of Helicoverpa armigera acyl-CoA delta-9 desaturase gene
摘要
Abstract
In the present study acyl-CoA delta-9 desaturase Cdna was obtained from Helicoverpa ar-migera by RT-PCR and RACE methods. According to the sequence analysis it was found that the full-length sequence of acyl-CoA delta-9 desaturase Cdna contains 2 931 nucleotides and the open reading frame contain 1 062 s nucleotides encoding 33 amino acid residues with predicted 40.66 Kd molecular weight. Developmentally expressed profile found that this gene arrived peak in early of 5th larvae, and was down-regulated by hunger and juvenile hormone, further molting hormone, 20E, challenge found that acyl-CoA delta-9 desaturase was up-regulated by 20E.关键词
棉铃虫/去饱和酶/分子鉴定/保幼激素/蜕皮激素Key words
Helicoverpa armigera/desaturase/molecular identification/juvenile hormone/molting hormone分类
生物科学引用本文复制引用
陈丽君,杜孟芳,安世恒,张松斗,蒋金炜..棉铃虫酰基辅酶A△9去饱和酶基因的分子鉴定[J].河南农业大学学报,2011,45(5):552-557,6.基金项目
河南省教育厅自然科学研究计划项目(2011B180021) （2011B180021）
