北京大学学报（医学版）2011，Vol.43Issue(6)：792-797,6.DOI:10.3969/i.issn.1671-167X.2011.06.002
缺氧诱导因子-1α及其靶基因在双胎输血综合征胎盘组织中的表达
Expressions of HIF-α and its target gene in monochorionic twin placentas with twintwin transfusion syndrome
杨玲玲 1邵珲 1原鹏波 1郭晓玥 1张小为 1赵扬玉1
作者信息
- 1. 北京大学第三医院妇产科,北京100191
- 折叠
摘要
Abstract
Objective: To determine hypoxia-inducible factor 1 a ( HIF-1α) and its target gene, vascular endothelial growth factor ( VEGF) and receptor ( VEGFR-1) concentrations in the placentas of the donor and recipient in monochorionic twin pregnancies with twin-twin transfusion syndrome ( TTTS). Methods: Twenty monochorionic twin pregnancy cases were included in the study (10 with and 10 without TTTS). Tissue protein expressions of HIF-1α,VEGF and VEGFR-1 were determined by using immu-nohistochemistry. Western blot analysis were used to quantify and compare the protein expression. RT-PCR were used to compare their mRNA expressions. Results: HIF-1α was mainly observed in tropho-blastic cells and villi capillaries endothelial cells, and VEGF in trophoblastic cells, endothelial cells and villi stromal cells; VEGFR-1 was mainly observed in villi trophoblastic cells and vascular endothelial cells. The placenta protein and mRNA expression of HIF-1α and its target gene in the donor placenta increased significantly (P＜0. 001) compared with that in the control placenta, but the expression of HIF-1α and its target gene in the recipients tended to be similar in the controls (P ＞0.05). There was no difference between the controls. Conclusion: When the monochorionic twin placenta is formed in the early period, HIF-1α, VEGF and VEGFR-1 are over-expressed, which may affect the placenta angiogenesis and induce TTTS .关键词
双胎输血综合征/缺氧诱导因子1,α亚基/血管内皮细胞生长因子/受体,血管内皮细胞生长因子Key words
Fetofetal transfusion/Hypoxia-inducible factor 1, alpha subunit/Vascular endothelial growth factor/Receptors, vascular endothelial growth factor分类
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杨玲玲,邵珲,原鹏波,郭晓玥,张小为,赵扬玉..缺氧诱导因子-1α及其靶基因在双胎输血综合征胎盘组织中的表达[J].北京大学学报（医学版）,2011,43(6):792-797,6.
