北京大学学报（医学版）2011，Vol.43Issue(6)：855-860,6.DOI:10.3969/j.issn.1671-167X.2011.06.014
Periostin酸性环境下对人脐静脉内皮细胞功能的影响
Effect of periostin on the function of human umbilical vein endothelial cells in acidic environment
摘要
Abstract
Objective:To observe the effect of recombinant periostin protein on the proliferation, apop-tosis and migration of human umbilical vein endothelial cells ( HUVECs) in acidic environment. Methods: HUVECs were divided into three groups; normal control ( pH 7. 31 ±0. 03) , acidic control (pH 6.95 ±0. 05) and experimental groups [ (periostin 100 μg/L, ( pH 6. 95 ±0. 05) ]. The cellular proliferation,apoptosis and migration were measured by Cell Counting Kit-8(CCK-8) assay, Annexin V-FITC with Flow Cytometer( at 60 h after periostin was given) and Transwell chamber assay (at 20 h after periostin was given). Results: In CCK-8 assay, the optical density values D450nm (at 72 h after periostin was given) of normal control, acidic control and experimental groups were 0.71 ±0.05, 0.62 ±0.04, 0.69 ±0.02, respectively. In the same order, the total apoptotic rate (%) of the three groups were 13. 06 ± 1. 35 , 16. 95 ± 0. 46, 12. 97 ± 1. 60, respectively; the numbers of migrated cells of the three groups were 67.45 ±11. 88,44. 89 ± 8. 67,64. 60 ± 9. 63 , respectively. Compared with normal control, the D450nm value and the number of migrated cells of acidic control reduced 12. 68% (P ＜0. 01) and 33.44% (P＜0.01) respectively. Otherwise, the total apoptotic rate (%) increased by 29. 79% (P＜ 0. 05). Compared with acidic control, the D450nm value and the number of migrated cells of experimental group increased by 11. 29% ( P ＜0. 01) and 43. 90% ( P ＜0. 01 ) , respectively. Meantime, the total apoptotic rate (%) reduced 23.48% (P＜0.05). Conclusion: In acidic environment, the HUVECs' abilities of proliferation and migration were lower; while, the apoptotic value was raised. The recombinant periostin protein could counteract the acidic influence by enhancing the cellular abilities of proliferation and migration. Periostin could make endothelial cells reisist acidosis and keep growing to form blood vessels so that the local histiocytes could get enough nutrients for survival.关键词
Periostin/内皮细胞/增殖/凋亡/迁移Key words
Periostin/Endothelial cells/Proliferation/Apoptosis/Migration分类
医药卫生引用本文复制引用
陈鑫磊,边曦,秦泽莲..Periostin酸性环境下对人脐静脉内皮细胞功能的影响[J].北京大学学报（医学版）,2011,43(6):855-860,6.基金项目
国家自然科学基金(30973126)资助 （30973126）
