西安交通大学学报（医学版）2011，Vol.32Issue(5)：610-614,5.
抗氧化剂吡咯烷二硫代氨基甲酸有助于提高卡铂对宫颈癌SiHa细胞化疗的敏感性
Pyrrolidine dithiocarbamate helps improve the chemosensitivity of cervical cancer cell SiHa to carboplatin
摘要
Abstract
Objective To study the effect of antioxidant pyrrolidine dithiocarbamate (PDTC) in promoting the chemosensitivity of human cervical cancer cell line SiHa to carboplatin and the possible mechanisms. Methods Human cervical carcinoma SiHa cell lines were cultured in vitro. They were treated with different concentrations of PDTC, carboplatin or PDTC combined with carboplatin for different durations. Then the inhibition of SiHa cells growth was detected by MTT assay; flow cytometry was used to analyze the apoptosis and cell cycle. The cellular distribution and protein level of NF-κBp65 were detected by immunocytochemical staining. Results PDTC could significantly inhibit the proliferation of SiHa cells in time-dependent and dose-dependent manners (P<0.01). Compared with carboplatin alone, PDTC (12.5 μmol/L) combined with carboplatin could significantly inhibit the proliferation of SiHa cells (P<0.01). The apoptosis rate of SiHa cell differed significantly between the drug group and the control group (P<0.01); the difference between PDTC + carboplatin combination group and carboplatin alone group was significant (P<0. 01). PDTC promoted G0/G1 phase of cell cycle arrest while carboplatin promoted G2/M phase of cell cycle arrest. Interestingly, G2/M phase was further arrested when carboplatin was added. The cells of S phase were also decreased. NF-κBp65 was mainly located in the cytoplasm of SiHa cell in the control group. The expression of NF-κBp65 in SiHa cell was transferred from cytoplasm to nucleus after 24-hour treatment with carboplatin, which increased the activity, whereas PDTC could inhibit the activity. Conclusion The activity of NF-κB p65 in SiHa cell was increased when treated with carboplatin. Even at a lower concentration, carboplatin combined with PDTC (12.5 μmol/L) resulted in a more significant growth inhibition and apoptosis of SiHa cell than carboplatin alone. PDTC can increase the chemosensitivity of cervical cancer SiHa cell to carboplatin.关键词
富颈肿瘤/卡铂/吡咯烷二硫代氨基甲酸/核转录因子κB/凋亡Key words
cervical cancer/carboplatin/pyrrolidine dithiocarbamate (PDTC)/nuclear factor kappa B/apoptosis分类
医药卫生引用本文复制引用
闫亚妮,吴静,张颖,徐丹,潘荣,刘变利..抗氧化剂吡咯烷二硫代氨基甲酸有助于提高卡铂对宫颈癌SiHa细胞化疗的敏感性[J].西安交通大学学报（医学版）,2011,32(5):610-614,5.基金项目
陕西省科技攻关资助项目(No.2008K09-08) （No.2008K09-08）
